Medical Applications of Mass Spectrometry

Cover

Contents

List of Contributors

Preface

Part I: Motivation and Essentials

Chapter 1. Introduction

Chapter 2. Basics of analytical chemistry and mass spectrometry for medical professionals

1. Introduction

2. Terms and definitions

3. The analytical procedure

4. A case study: analysis of plasma sterol profile

5. Mass spectrometry

Reference

Chapter 3. Ethical, legal, safety, and scientific aspects of medical research

1. Ethical aspects

2. Legal aspects

3. Safety aspects

4. Handling biological materials

5. Clinical trials and protocols

6. Administrative procedures

References

Part II: Tools of the Trade

Chapter 4. Biomedical sampling

1. Sampling

2. Sample preparation

3. Extraction techniques

4. Automation and high throughput

5. Outlook

References

Chapter 5. Separation methods

1. Introduction

2. Gas chromatography

3. High-performance liquid chromatography

4. Electrophoretic techniques

5. Future trends

References

Chapter 6. Mass spectrometry instrumentation and techniques

1. Introduction

2. General questions about mass measurement and mass spectrometry

3. Separation techniques: gas chromatography (GC), and high-performance liquid chromatography (HPLC)

4. Ionization methods

5. Mass analyzers

6. Tandem mass spectrometry (MS/MS)

7. Selected terms for clarification

Acknowledgment

References

Chapter 7. Chemoinformatics„multivariate mathematical.statistical methods for data evaluation

1. Introduction

2. Data types and data pretreatment

3. Multivariate methods

4. Selected applications of chemometrics

Terms and terminology

References

Part III: Biomolecules

Chapter 8. Mass spectrometry in proteomics

1. Introduction

2. Methods in proteomics

3. Outlook

References

Chapter 9. De novo sequencing of peptides

Chapter 10. Protein bioinformatics

1. Highlights for medical professionals

2. Introduction

3. Methodology

4. Discussion

5. Future trends

6. Conclusions

Acknowledgments

References

Chapter 11. Analysis of complex lipidomes

1. Introduction

2. Methodology

3. Phospholipids

4. Acylglycerols

5. Sphingolipids

6. Sterols

7. Medical applications of MS-lipidomics

8. Future trends

9. Conclusions

References

Part IV: Selected Medical Applications

Chapter 12. Medical laboratory practice„possibilities for mass spectrometry

1. Introduction

2. Quality management

3. Inborn errors of metabolism

4. Assessment of in vivo metabolism using stable isotope techniques

5. Conclusions

References

Chapter 13. Therapeutic drug monitoring and measurement of drug concentrations using mass spectrometry

1. Introduction

2. Antiinfection drugs

3. Drugs acting on the central nervous system (CNS)

4. Cardiovascular drugs

5. Anticancer agents

6. Analgesics

7. Miscellaneous drug classes

References

Chapter 14. Mass spectrometry of infectious pathogens

1. Introduction

2. Methodology

3. Future trends and prospects

References

Chapter 15. Proteomics of viruses

1. Introduction

2. Virus–host interactions

3. Diagnostics

4. Discussion

5. Future trends

6. Conclusions

Acknowledgments

References

Chapter 16. Neonatal research

1. Introduction

2. Principle of the methodology

3. Quick reference for practical implementation of the methodology

4. Key points of the methodology

5. Ongoing extensions of NBS

6. Conclusions

Acknowledgments

References

Chapter 17. Applications of mass spectrometry in oncology

1. Introduction

2. New methodology„SELDI.TOF.MS

3. Other relevant methodological challenges

4. Diagnostic oncoproteomics based on SELDI–TOF–MS

5. Representative other applications

References

Chapter 18. Application of mass spectrometry in brain research: neurotransmitters, neuropeptides, and brain proteins

1. Introduction

2. Methodology

3. Discussion

4. Future trends

5. Conclusions

Acknowledgments

References

Chapter 19. The human pituitary proteome: clinical applications

1. Introduction

2. The pituitary gland and mass spectrometry: an endocrinologist’s perspective

3. Methodology

4. Discussion

5. Future trends

6. Conclusions

Acknowledgments

References

Chapter 20. Mass spectrometry of proteinous allergens inducing human diseases

1. Introduction

2. Methodology

3. Mass spectrometric identification of proteinous allergens

4. Future trends

5. Conclusions

References

Chapter 21. Mass spectrometry in clinical treatment

1. Introduction

2. Pediatrics

3. Oncology

References

Part V: Emerging Areas

Chapter 22. Biomarker discovery

1. Introduction

2. Biomarkers in medicine

3. Important definitions

4. Biomarkers discovery and complexity of biological systems

5. Biomarker discovery and omicsŽ

6. The biomarker discovery project

7. Challenges in the biomarker discovery pathway

8. Technical issues in experimental design

9. The discovery process overview

10. Conclusions

References

Chapter 23. Molecular imaging by mass spectrometry

1. Introduction

2. Methods

3. Discussion

4. Future trends

5. Conclusion

Acknowledgments

References

Chapter 24. Brief outlook

1. Trends in instrumentation

2. Emerging systems approach

3. Mass spectrometry in translational medicine

Index